I don't know about the computer teams doing it mid series... but I do know humans can make DL activations on one day between each series in OOTP3. However if you miss the day you are screwed. You also are not allowed to promote anyone from AAA at any time during the postseason... so if you decide to keep an extra hitter for the wild card round hoping to replace him with that extra pitcher for the LCS you won't be able to do that... unless you put the extra pitcher on the DL before the post season... and he becomes eligible before that magic day when the game will allow you to activate players.
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